"With the mitochondrial analysis completed, the team is working on the nuclear DNA analysis and comparing the samples to paternal relatives of the czar's family."
I assume they will be comparing the Y-chromosome DNA (if they find it) to that of Nicholas, but they might as well compare it with living relatives from Nicholas' side of the family as well...
That's why this doesn't make sense. If they do the Y chromosome test, which is when N's relatives may or may not need to come into the picture, then that wouldn't work for the female remains. If they do the nuclear DNA paternity tests, then there is no need to do the Y chromosome test (which is a lot more complicated from what I understand), and why would the relatives need to be involved? It doesn't really make sense either way.
Well, the Y chromosome testing is the only way to prove it's Alexei's remains imo. So they definately have to do that.
And to prove the other remains belong to the missing GD, they'll have to do nDNA testing on the female remains as well.
I was just thinking, did they ever find nDNA on the first remains? mtDNA is far easier to have survived (since there's far more of it) than nDNA.
If they have no nDNA from Nicholas, they'll have to compare it with relatives ofc.
Another reason to involve living relatives might be to just have an extra body of evidence. The more evidence the better imo.
People could start questioning the initial DNA tests or the initial remains or whatever (as some most probably already have and always will). But then again, if that evidence didn't suffice, what would...
I also really hope they'll be able to prove the presence of the faulty factor VIII gene.
Do they know which gene was flawed in the Victoria-Alexandra hemophilia line?
I don't think they will be doing that, as it is redundant. They didn't do it for the other remains because it was not necessary, and I doubt they will do it for these. All they need to do is a paternity test to see if the nDNA matches the parentage of N&A's nDNA, and that will be proof enough. That should also show that the female remains are different from the other 3 younger female remains, and for the male remains, as long as nDNA and mtDNA match and the female remains match, nothing else is necessary.
As far as which gene was flawed, it would be the one that codes for Factor VIII protein of course.
Well, that's what I was asking. Why is it so sure it would be Factor VIII? As far as I know the seperation between Hemophilia A and B (Factor VIII fault, Factor IX fault respectively) can only be determined for sure through DNA testing. You could make a conclusion based on symptoms and severity, but that's not 100% conclusive I would imagine.
And it's not needed indeed and some might call it redundant. But I think it would be interesting to prove that Alexei's condition was indeed Hemophilia A (which is something some people still don't want to believe).
It's not needed to prove any identity or something, but why not test it just for the sake of knowing?
Grtz,
Oliver